Quantalase has developed Fluorimeters which use banks of pulsed LEDs to excite fluorescence in sample under study. The wavelength, pulse duration and peak power of the LED output can be set to match the excitation requirements of the sample. The fluorescence is detected by a pulsed photomultiplier. Suitable filters after the LEDs and before the photomultiplier tube prevent LED light from reaching the photomultiplier tube directly. The filters can be broad band coloured glass filters or multilayer narrow band filters. The instrument is controlled by a microcontroller which pulses the LEDs and photomultiplier tube. The microcontroller also controls the ADC which convert the fluorescence signal from photomultiplier to digital form for further processing. A single board computer averages the photomultiplier output over 2000 pulses and carries out any calculations necessary.A touch screen display permits the operator to set necessary parameters and also display the fluorescence measurement.
General specifications of Fluorimeter
- Detecter - Photomultiplier tube
- Excitation source - LEDs
- Wavelength of emission - As required by the user. Wavelength can be selected between 200nm to 500 nm.
- Bandwidth of emission - Typically 10-20 nm.
- Pulse duration - Between 2 to 50 microsecs. To be set by operater.
- Pulse repetition rate - 1 KHzs.
